Experience with the BIOMED-2 standardized polymerase chain reaction protocol for immunoglobulin and T- cell receptor clonality analysis in the Instituto Nacional de Cancerología, Colombia
Abstract
Introduction: The European BIOMED-2 consortium was created to evaluate clonality in lymphoproliferations that are difficult to diagnose. In Colombia, the implementation of these tests began in 2015 at the Instituto Nacional de Cancerología E.S.E., Bogotá.
Objectives: To determine the behavior of the rearrangement tests for lymphoid clonality and the difficulties of its implementation in our field through a series of retrospective and consecutive cases of lymphoid proliferation subjected to the BIOMED-2 protocols.
Materials and methods: Clinical and histological data and the results of the rearrangement analysis of all cases of lymphoid proliferation subjected to the BIOMED-2 protocols between February 2015 and May 2019 were collected from clinical histories.
Results: We recovered 132 samples from which 47 corresponded to reactive lymphoid hyperplasias, 62 to T lymphomas, 19 to B lymphomas, and three to lymphoid neoplasms of unestablished lineage. Only in one case did DNA extraction fail. According to these results, the greatest diagnostic difficulty for the pathologist was the analysis of T lymphoid infiltrates, most of which (44) were skin lesions.
Conclusions: Clonality tests can be used in tissues of different quality to help in the diagnosis of lymphoid proliferations that are difficult to classify. It is important to implement and interpret them in an interdisciplinary way considering each case separately.
Downloads
References
Instituto Nacional de Cancerología. Anuario estadístico 2010. 1a edición. Bogotá: Buenos y creativos S.A.S.; 2012.
Basso K, Dalla-Favera R. Germinal centres and B cell lymphomagenesis. Nat Rev Immunol. 2015;15:172-84. https://doi.org/10.1038/nri3814
Instituto Nacional de Cancerología. Anuario Estadístico 2015. 1a edición. Bogotá: Buenos y creativos S.A.S.; 2018.
Sandberg Y, van Gastel-Mol EJ, Verhaaf B, Lam KH, van Dongen JJM, Langerak AW, et al. BIOMED-2 multiplex immunoglobulin/T-cell receptor polymerase chain reaction protocols can reliably replace southern blot analysis in routine clonality diagnostics. J Mol Diagnostics. 2005;7:495-503. https://doi.org/10.1016/S1525-1578(10)60580-6
Hoeve MA, Krol AD, Philippo K, Derksen PW, Veenendaal RA, Schuuring E, et al. Limitations of clonality analysis of B cell proliferations using CDR3 polymerase chain reaction. Mol Pathol. 2000;53:194-200. https://doi.org/10.1136/mp.53.4.194
Langerak AW, Groenen PJ, Brüggemann M, Beldjord K, Bellan C, Bonello L, et al. EuroClonality/BIOMED-2 guidelines for interpretation and reporting of Ig/TCR clonality testing in suspected lymphoproliferations. Leukemia. 2012;26:2159-71.
Abbas AK, Lichtman AH, Pillai S. Cellular and molecular immunology, 8th edition. Amsterdam: Elsevier; 2014. p. 544.
Malcolm TIM, Hodson DJ, Macintyre EA, Turner SD. Challenging perspectives on the cellular origins of lymphoma. Open Biol. 2016;6:160232. https://doi.org/10.1098/rsob.160232
Kim Y, Choi YD, Choi C, Nam J-H. Diagnostic utility of a clonality test for lymphoproliferative diseases in Koreans using the BIOMED-2 PCR assay. Korean J Pathol. 2013;47:458-65. https://doi.org/10.4132/KoreanJPathol.2013.47.5.458
Langerak AW, Molina TJ, Lavender FL, Pearson D, Flohr T, Sambade C, et al. Polymerase chain reaction-based clonality testing in tissue samples with reactive lymphoproliferations: Usefulness and pitfalls. A report of the BIOMED-2 Concerted Action BMH4-CT98-3936. Leukemia . 2007;21:222-9. https://doi.org/10.1038/sj.leu.2404482
Davey DD, Kamat D, Zaleski S, Goeken J, Dick FR. Analysis of immunoglobulin and T-cell receptor gene rearrangement in cytologic specimens. Acta Cytol. 1989;33:583-90.
Davis TH, Yockey CE, Balk SP. Detection of clonal immunoglobulin gene rearrangements by polymerase chain reaction amplification and single-strand conformational polymorphism analysis. Am J Pathol. 1993;142:1841-7.
Trainor KJ, Brisco MJ, Wan JH, Neoh S, Grist S, Morley AA. Gene rearrangement in B- and T-lymphoproliferative disease detected by the polymerase chain reaction. Blood. 1991;78:192-6.
van Dongen JJM, Langerak AW, Brüggemann M, Evans PAS, Hummel M, Lavender FL, et al. Design and standardization of PCR primers and protocols for detection of clonal
immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: Report of the BIOMED-2 concerted action BMH4-CT98-3936. Leukemia. 2003;17:2257-317. https://doi.org/10.1038/sj.leu.2403202
Langerak AW, Molina TJ, Lavender FL, Pearson D, Flohr T, Sambade C, et al. Polymerase chain reaction-based clonality testing in tissue samples with reactive lymphoproliferations: Usefulness and pitfalls. A report of the BIOMED-2 Concerted Action BMH4-CT98-3936. Leukemia. 2007;21:222-9. https://doi.org/10.1038/sj.leu.2404482
Evans PA, Pott C, Groenen PJ, Salles G, Davi F, Berger F, et al. Significantly improved PCR-based clonality testing in B-cell malignancies by use of multiple immunoglobulin gene targets. Report of the BIOMED-2 Concerted Action BHM4-CT98-3936. Leukemia. 2007;21:207-14. https://doi.org/10.1038/sj.leu.2404479
Brüggemann M, White H, Gaulard P, García-Sanz R, Gameiro P, Oeschger S, et al. Powerful strategy for polymerase chain reaction-based clonality assessment in T-cell malignancies Report of the BIOMED-2 Concerted Action BHM4 CT98-3936. Leukemia. 2007;21:215-21. https://doi.org/10.1038/sj.leu.2404481
Groenen PJTA, Langerak AW, van Dongen JJM, van Krieken JHJM. Pitfalls in TCR gene clonality testing: Teaching cases. J Hematop. 2008;1:97-109. https://doi.org/10.1007/s12308-008-0013-9
Schuurhuis GJ, Heuser M, Freeman S, Béne MC, Buccisano F, Cloos J, et al. Minimal/measurable residual disease in AML: A consensus document from the European LeukemiaNet MRD Working Party. Blood. 2018;131:1275-91. https://doi.org/10.1182/blood-2017-09-801498
Lassmann S, Gerlach U V, Technau-Ihling K, Werner M, Fisch P. Application of BIOMED-2 primers in fixed and decalcified bone marrow biopsies: Analysis of immunoglobulin H receptor rearrangements in B-cell non-Hodgkin’s lymphomas. J Mol Diagn. 2005;7:582-91. https://doi.org/10.1016/S1525-1578(10)60591-0
Berget E, Helgeland L, Molven A, Vintermyr OK. Detection of clonality in follicular lymphoma using formalin-fixed, paraffin-embedded tissue samples and BIOMED-2 immunoglobulin primers. J Clin Pathol. 2011;64:37-41. https://doi.org/10.1136/jcp.2010.081109
Zhang S, Abreo F, Lowery-Nordberg M, Veillon DM, Cotelingam JD. The role of fluorescence in situ hybridization and polymerase chain reaction in the diagnosis and classification of lymphoproliferative disorders on fine-needle aspiration. Cancer Cytopathol. 2010;118:105-12. https://doi.org/10.1002/cncy.20070
Dawidowska M, Jółkowska J, Szczepański T, Derwich K, Wachowiak J, Witt M. Implementation of the standard strategy for identification of Ig/TCR targets for minimal
residual disease diagnostics in B-cell precursor ALL pediatric patients: Polish experience. Arch Immunol Ther Exp (Warsz). 2008;56:409-18. https://doi.org/10.1007/s00005-008-0045-y
Burack WR, Laughlin TS, Friedberg JW, Spence JM, Rothberg PG. PCR assays detect B-lymphocyte clonality in formalin-fixed, paraffin-embedded specimens of classical Hodgkin lymphoma without microdissection. Am J Clin Pathol. 2010;134:104-11. https://doi.org/10.1309/AJCPK6SBE0XOODHB
Payne K, Wright P, Grant JW, Huang Y, Hamoudi R, Bacon CM, et al. BIOMED-2 PCR assays for IGK gene rearrangements are essential for B-cell clonality analysis in follicular lymphoma. Br J Haematol. 2011;155:84-92. https://doi.org/10.1111/j.1365-2141.2011.08803.x
Sandberg Y, Heule F, Lam K, Lugtenburg PJ, Wolvers-Tettero ILM, van Dongen JJM, et al. Molecular immunoglobulin/T- cell receptor clonality analysis in cutaneous lymphoproliferations. Experience with the BIOMED-2 standardized polymerase chain reaction protocol. Haematologica. 2003;88:659-70.
Tan BT, Seo K, Warnke RA, Arber DA. The frequency of immunoglobulin heavy chain gene and T-cell receptor γ-chain gene rearrangements and Epstein-Barr virus in ALK+ and ALK− anaplastic large cell lymphoma and other peripheral T-cell lymphomas. J Mol Diagn. 2008;10:502-12. https://doi.org/10.2353/jmoldx.2008.080054
Sufficool KE, Lockwood CM, Abel HJ, Hagemann IS, Schumacher JA, Kelley TW, et al. T-cell clonality assessment by next-generation sequencing improves detection sensitivity in mycosis fungoides. J Am Acad Dermatol. 2015;73:228-236.e2. https://doi.org/10.1016/j.jaad.2015.04.030
van Dongen JJM, Langerak AW, Brüggemann M, Evans PAS, Hummel M, Lavender FL, et al. Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: Report of the BIOMED-2 Concerted Action BMH4-CT98-3936. Leukemia. 2003;17:2257-317. https://doi.org/10.1038/sj.leu.2403202
Gazzola A, Mannu C, Rossi M, Laginestra MA, Sapienza MR, Fuligni F, et al. The evolution of clonality testing in the diagnosis and monitoring of hematological malignancies. Ther Adv Hematol. 2014;5:35-47. https://doi.org/10.1177/2040620713519729
Kokovic I, Novakovic BJ, Cerkovnik P, Novakovic S. Clonality analysis of lymphoid proliferations using the BIOMED-2 clonality assays: A single institution experience. Radiol Oncol. 2014;48:155-62. https://doi.org/10.2478/raon-2013-007
Rout ED, Burnett RC, Yoshimoto JA, Avery PR, Avery AC. Assessment of immunoglobulin heavy chain, immunoglobulin light chain, and T-cell receptor clonality testing in the diagnosis of feline lymphoid neoplasia. Vet Clin Pathol. 2019;48 (Supl.1):45-58. https://doi.org/10.1111/vcp.12767
Suefuji N, Niino D, Arakawa F, Karube K, Kimura Y, Kiyasu J, et al. Clinicopathological analysis of a composite lymphoma containing both T- and B-cell lymphomas. Pathol Int. 2012;62:690-8. https://doi.org/10.1111/j.1440-1827.2012.02858.x
Some similar items:
- Jorge de Jesús Cantillo, Rocío del Pilar López, Rafael Enrique Andrade, Glomerular deposition disease, regarding a case of fibrillary glomerulonephritis , Biomedica: Vol. 29 No. 4 (2009)
- Carolina Guerra, Carlos Santiago Uribe, Alejandro Guerra, Olga H. Hernández, Bickerstaff brain encephalitis: case report and review , Biomedica: Vol. 33 No. 4 (2013)
- Edwin Abraham Medina, Rocío Orduz, Olga Lucía Morales, Óscar Martínez, Margarita Baldión, Mario Arturo Isaza, Adult T-cell leukemia/lymphoma in HTLV-1 infected patients: report of two cases in Colombia , Biomedica: Vol. 33 No. 4 (2013)
- Alejandra Catalina Vélez, Diana María Castaño, Rubén Darío Gómez, Julio César Orrego, Marcela Moncada, José Luis Franco, Common variable immunodeficiency: Clinical and immunological characterization of patients and homogeneous subgroup definition by means of B lymphocyte subpopulation typing , Biomedica: Vol. 35 No. 1 (2015)
- Diana L. Franco, Sameer Islam, Kevin Ruff, Endoscopic findings of primary follicular lymphoma , Biomedica: Vol. 35 No. 1 (2015)
- Nhora Silva, Natalia Calvache, Elizabeth Arrieta, Juan Carlos Bravo, Camilo Salazar, Joaquín Rosales, Francisco Jaramillo, Juan Guillermo Restrepo, Disseminated histoplasmosis as a first clinical manifestation in a patient with small lymphocytic lymphoma: A case report , Biomedica: Vol. 38 No. 3 (2018)
- María Adoración Martín-Gómez, Mercedes Caba-Molina, María José Viciana-Martínez-Lage, Rosa Ortega-Salas, Alicia Sánchez-Crespo, Manuel Jesús Soriano-Pérez, Mercedes Gómez-Morales, Collapsing glomerulonephritis with podocyte markers in hemophagocytic syndrome secondary to hepatosplenic T-cell lymphoma , Biomedica: Vol. 38 No. 4 (2018)
- Leonardo F. Jurado, Andrés Gómez-Aldana, Mónica Tapias, Daniela Cáceres, Alonso Vera, Rocío del Pilar López-Panqueva, Rafael E. Andrade, Post-transplant lymphoproliferative disorders in a cohort of adult patients with a liver transplant from a reference hospital in Bogotá, Colombia , Biomedica: Vol. 40 No. 3 (2020)
Article metrics | |
---|---|
Abstract views | |
Galley vies | |
PDF Views | |
HTML views | |
Other views |